cellmask™ deep red stain Search Results


mitotracker deep red staining kit  (Keygen Biotech)
90
Keygen Biotech mitotracker deep red staining kit
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Mitotracker Deep Red Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mitotracker deep red staining kit/product/Keygen Biotech
Average 90 stars, based on 1 article reviews
mitotracker deep red staining kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cellmask deeptm red cytoplasmic/nuclear stain  (Promega)
90
Promega cellmask deeptm red cytoplasmic/nuclear stain
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Cellmask Deeptm Red Cytoplasmic/Nuclear Stain, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellmask deeptm red cytoplasmic/nuclear stain/product/Promega
Average 90 stars, based on 1 article reviews
cellmask deeptm red cytoplasmic/nuclear stain - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
deep red membrane stain  (Chroma Technology Corporation)
90
Chroma Technology Corporation deep red membrane stain
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Deep Red Membrane Stain, supplied by Chroma Technology Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deep red membrane stain/product/Chroma Technology Corporation
Average 90 stars, based on 1 article reviews
deep red membrane stain - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lipi-deep red neutral lipid stain  (Dojindo Labs)
90
Dojindo Labs lipi-deep red neutral lipid stain
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Lipi Deep Red Neutral Lipid Stain, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipi-deep red neutral lipid stain/product/Dojindo Labs
Average 90 stars, based on 1 article reviews
lipi-deep red neutral lipid stain - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
dylight 649/did/cellmask deep red plasma membrane stain  (DPSS Lasers)
90
DPSS Lasers dylight 649/did/cellmask deep red plasma membrane stain
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Dylight 649/Did/Cellmask Deep Red Plasma Membrane Stain, supplied by DPSS Lasers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dylight 649/did/cellmask deep red plasma membrane stain/product/DPSS Lasers
Average 90 stars, based on 1 article reviews
dylight 649/did/cellmask deep red plasma membrane stain - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cellmask deep red plasma membrane stain  (Carl Zeiss)
90
Carl Zeiss cellmask deep red plasma membrane stain
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Cellmask Deep Red Plasma Membrane Stain, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellmask deep red plasma membrane stain/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
cellmask deep red plasma membrane stain - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
live or dead fixable dead cell staining kit [deep red fluorescence]  (AAT Bioquest)
90
AAT Bioquest live or dead fixable dead cell staining kit [deep red fluorescence]
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Live Or Dead Fixable Dead Cell Staining Kit [Deep Red Fluorescence], supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/live or dead fixable dead cell staining kit [deep red fluorescence]/product/AAT Bioquest
Average 90 stars, based on 1 article reviews
live or dead fixable dead cell staining kit [deep red fluorescence] - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lysotracker green, tubulin-tracker deep red staining kit for living cells  (Beyotime)
90
Beyotime lysotracker green, tubulin-tracker deep red staining kit for living cells
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Lysotracker Green, Tubulin Tracker Deep Red Staining Kit For Living Cells, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysotracker green, tubulin-tracker deep red staining kit for living cells/product/Beyotime
Average 90 stars, based on 1 article reviews
lysotracker green, tubulin-tracker deep red staining kit for living cells - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cellmask™ deep red plasma membrane stain  (LC Laboratories)
90
LC Laboratories cellmask™ deep red plasma membrane stain
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Cellmask™ Deep Red Plasma Membrane Stain, supplied by LC Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellmask™ deep red plasma membrane stain/product/LC Laboratories
Average 90 stars, based on 1 article reviews
cellmask™ deep red plasma membrane stain - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
schiffs reagent-glycogen  (Merck & Co)
90
Merck & Co schiffs reagent-glycogen
FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of <t>Mitotracker</t> Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).
Schiffs Reagent Glycogen, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/schiffs reagent-glycogen/product/Merck & Co
Average 90 stars, based on 1 article reviews
schiffs reagent-glycogen - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).

Journal: Scientific Reports

Article Title: Florfenicol-induced Mitochondrial Dysfunction Suppresses Cell Proliferation and Autophagy in Fibroblasts

doi: 10.1038/s41598-017-13860-9

Figure Lengend Snippet: FLO inhibits Parkin-mediated mitochondrial autophagy and promotes cell senescence. ( A ) Immunoblot analysis of autophagy-related proteins in L cells. Cells were treated with 0.1 mg/mL FLO for 12 h or 24 h, and combined treatment with Baf A1 (200 nM) as indicated. GAPDH served as a loading control. Densitometry was performed for quantification and the ratios of LC3B-II or p62 to GAPDH are presented at the bottom of the blots, respectively. Cropped blots are displayed and full-length blots are included in the Supplementary Information file. ( B ) Cox IV and Tim23 levels were detected by western blot with the whole cell lysis extracted form L cells. Cells were treated with FLO for 12 h or 24 h, or were pretreated with FLO for 8 h of 20 h and then co-treated with CCCP (100uM) for another 4 h as indicated. ( C ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells treated with FLO (0.1 mg/mL) for 24 h. The mean fluorescence of Mitotracker Deep Red represents the level of mitochondrial population. CCCP (100 uM for 4 h) was used as a positive control for the clearance of damaged mitochondria through mitophagy. ( D ) Representative images of SA β-gal staining in L cells in the presence of FLO (0.1 mg/mL) for eight days. The medium containing FLO was renewed every two days. SA β-gal-positive cells were quantified (n = 3). ( E ) Immunoblots show levels of the Pink1, recruited-Parkin and p62 on mitochondrial outer membrane in L cells. Cells were treated with FLO (0.1 mg/mL) only or co-treated with FLO and NAC (2 mM) for 24 h. CCCP (100 uM for 4 h) was used as a positive control for the induction of mitophagy through Pink1-Parkin pathway. Cox IV served as a loading control. ( F ) Flow cytometric analysis of Mitotracker Deep Red fluorescence in L cells that co-treated with FLO (0.1 mg/mL) and NAC (2 mM) for 24 h. Histograms in the figure represents the mean ± SD. **p < 0.01, as compared with the indicated group (LSD multiple comparison test after one-way ANOVA analysis).

Article Snippet: The apoptosis rates, cell cycle distribution, mitochondrial membrane potential, ROS levels and mitochondrial mass were assessed with the Annexin V-PE/7-AAD staining kit (KeyGen Biotech, Nanjing, China), cell cycle analysis kit (Beyotime, Shanghai, China), JC-1 detection kit (KeyGen Biotech, Nanjing, China), ROS assay kit (Beyotime, Shanghai, China), and MitoTracker Deep Red staining kit (KeyGen Biotech, Nanjing, China), respectively.

Techniques: Western Blot, Control, Lysis, Fluorescence, Positive Control, Staining, Membrane, Comparison